Adequate Training Is Important to Enter the World of PhlebotomyAdequate training of
phlebotomist responsible for collecting blood and urine specimens is of utmost importance to assure
safety and avoid false results. Otherwise, an improperly collected sample will lead to false lab results and errors
that can harm the patient, or even cause their death. Almost certainly it will increase the cost of care. Only
through skillful and efficient blood drawing procedures can a quality blood sample be obtained and reliable results
be recorded.

Blood Sample Collection and Proper Techniques
A phlebotomist must understand that each person is different, each situation unique and a
routine situation can turn critical in the blink of an eye. No matter what the circumstances, potential dangerous
situations must be recognized and procedural errors prevented. Special challenges arise when
collecting blood from the antecubital fossa area is not possible because of bad veins, obesity, or the
presence of indwelling intravenous lines (IV), or when an arterial vessel must be accessed for arterial blood
gas tests and there is a vascular access device (VAD) in place. There is MUCH, MUCH more to proper blood sample
collection and lab test recording than meets the eye.
Other areas of concerns are...
 diurnal and circadian variations
 patient preparation
 patient general health and gender
 physiologic variables
 medications a patient is taking
 patient's position when drawing
 tourniquet placement and venous stasis
 puncture of a secondary site
 safety and infection control to prevent complications or injury to self and
others.
Required blood tests may include timed procedures for analysis, e.g. glucose tolerance and drug levels and
special tests such as STATS, blood culture, or tests required to be obtained in a fasting state. Some tests may
require administration of oral diagnostic medications such as glucose and xylose and will be given to patient per
the doctor's instructions.
The right blood drawing technique will...
- prevent errors (e.g. false lab results)
- prevent injuries (e.g. anticoagulant reflux, nerve injury)
- prevent compromised samples (e.g. hemolysis, hemoconcentration, or agglutination)
To assure blood specimen integrity the phlebotomist must:
 properly identify the patient
 give proper instructions
 secure and access the blood vessel
 correctly label the collection tubes
 use the right blood collection devices and tubes
 properly handle the collection tubes
 follow the right order of draw
 maintain the sample's integrity while drawing
 sufficiently fill the collection tubes
 properly process the blood sample
 properly invert the collection tubes
 properly record all information about the sample
 and properly store or transport the tubes to maintain specimen quality.
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